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Part:BBa_K136014

Designed by: alexandra bouaziz   Group: iGEM08_Paris   (2008-10-25)


AracpBAD - gfp triparts

pBad is an E. coli promoter that is tightly controlled by: 

*inducer: L-arabinose.

* repressor: AraC apparently acts as the repressor

one study concluded that arabinose can change the conformation of araC and prevent it from successfully binding to and repressing pBad. pBad is followed by a GFP tripart in our construction .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2030
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1865
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 665
    Illegal SapI site found at 1847


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